Description Specificity Reacts with Retinal Pigment Epithelium 65 (RPE65). On bovine RPE membranes the antibody recognizes a protein with a molecular weight of ~65 kDa. Immunogen Bovine RPE microsomal membranes. Application Anti-Retinal Pigment Epithelium 65 Antibody detects level of Retinal Pigment Epithelium 65 & has been published & validated for use in ELISA, IH, IP & WB. Research Category Neuroscience Research Sub Category Sensory & PNS Western blot: 1:5,000-1:20,000 on bovine RPE membranes using ECL. Suggested dilution buffer is TBS containing 10% calf serum, 0.25% T-20, 1M D-glucose with 10% glycerol. Suggested blocking buffer is TBS containing 2% BSA and 0.5% Tween 20. Preferred gel percentage is 10%. Immunohistochemistry on frozen tissue sections: 1:250-1:500 Immunoprecipitation: 20 μg of antibody in a reaction volume of 500 μL. Immunoaffinity purification ELISA Optimal working dilutions must be determined by end user. Target description ~ 65 kDa Physical form Format: Purified Protein A Purfied Purified immunoglobulin. Liquid in PBS. Contains no preservative. Storage and Stability Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Analysis Note Control Eye Other Notes Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.