Anti-Spectrin beta-II, clone 2A7

Description General description Spectrin beta (UniProt: G1SN11) is encoded by the SPTBN1 gene in rabbit. Spectrins belong to the superfamily of proteins called F-actin cross linking proteins that function as scaffolding proteins for protein sorting, cell adhesion, and migration. They are principle components of a cell membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. They are actin binding proteins that serves as a membrane organizer and stabilizer. Spectrin beta II is a multifunctional protein that contains lipid-binding sites within its two calponin-homology domains (aa 82-186 and 201-306) and a pleckstrin homology domain (aa 2224-2334) and triple helical segments. Spectrin beta II is generally associated with the cytoplasmic surface of the membrane by attachment to ankyrin. Specificity Clone 2A7 is a mouse monoclonal antibody that detects beta-II spectrin. Immunogen Purified spectrin from frozen rabbit lens membranes. Application Anti-Spectrin beta-II , clone 2A7, Cat. No. MABT1364, is a mouse monoclonal antibody that detects Spectrin beta chain and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting. Immunoprecipitation Analysis: A representative lot immunoprecipitated Spectrin beta-II in Immunoprecipitation applications (Bazellieres, E., et. al. (2012). J Cell Sci. 125(Pt 4):919-31). Immunocytochemistry Analysis: A representative lot detected Spectrin beta-II in Immunocytochemistry applications (Bazellieres, E., et. al. (2012). J Cell Sci. 125(Pt 4):919-31). Western Blotting Analysis: A representative lot detected Spectrin beta-II in Western Blotting applications (Bazellieres, E., et. al. (2012). J Cell Sci. 125(Pt 4):919-31). Research Category Cell Structure Target description ~275 kDa observed; 271.27 kDa calculated. Uncharacterized bands may be observed in some lysate(s). Physical form Format: Purified Protein G purified Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Storage and Stability Stable for 1 year at 2-8°C from date of receipt. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Quality Evaluated by Western Blotting in differentiated Caco2 cell lysates. Western Blotting Analysis: 2 µg/mL of this antibody detected Spectrin beta-II in differentiated Caco2 cell lysates. Other Notes Concentration: Please refer to lot specific datasheet.