Anti-TSLP Receptor, clone 22H9

Description General description Cytokine receptor-like factor 2 (UniProt: Q8CII9; also known as Cytokine receptor-like molecule 2, CRLM-2, Thymic stromal lymphopoietin protein receptor, TSLP receptor, Type I cytokine receptor delta 1) is encoded by the Crlf2 (also known as rlm2, Tpte2, Tslpr) gene (Gene ID: 57914) in murine species. TSLP receptor is a single-pass type I membrane protein that serves as a receptor for thymic stromal lymphopoietin. It is expressed in liver, lung, testis, heart, brain, spleen, thymus, and bone marrow. Higher expression levels are observed in progenitors and myeloid cells. Its levels can be up-regulated in the myeloid 32D cell line by granulocyte colony-stimulating factor. TSLP receptor forms a functional complex with TSLP and IL7R, which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. It has also been implicated in the development of the hematopoietic system. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form. The mature form contains an extracellular domain (aa 20-232), a transmembrane domain (aa 233-253), and a cytoplasmic domain (aa 254-359). It contains WSXWS motif (aa 201-205), which is essential for proper protein folding and efficient intracellular transport and cell-surface binding. It also has a Box 1 motif (aa 262-270) that is required for JAK interaction and activation. Specificity Clone 22H9 is a rat monoclonal antibody that detects Thymic stromal lymphopoietin protein receptor (TSLPR) in murine species. It targets an epitope with in the extracellular domain. Immunogen Epitope: extracellular domain His-tagged murine recombinant TSLP Receptor Protein. Application Anti-TSLP Receptor, clone 22H9, Cat. No. MABF1966, is a rat monoclonal antibody that detects TSLP receptor and has been tested for use in Flow Cytometry. Flow Cytometry Analysis: A representative lot detected TSLP receptor in Flow Cytometry applications (Bell, B.D., et. al. (2013). Nat Immunol. 14(4):364-71; Isaksen, D.E., et. al. (2002). J Immunol. 168(7):3288-94; Iseki, M., et. al. (2012). Int Immunol. 24(3):183-95; Kashiwagi, M., et. al. (2017). Nat Immunol. 18(3):334-343). Research Category Inflammation & Immunology Target description 37.76 kDa calculated. Physical form Format: Purified Protein G purified Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Storage and Stability Stable for 1 year at 2-8°C from date of receipt. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Quality Evaluated by Flow Cytometry in bone marrow cells from C57BL/6 Mouse. Flow Cytometry Analysis: 1 µg of this antibody detected TSLP receptor in one million bone marrow cells from C57BL/6 Mouse. Other Notes Concentration: Please refer to lot specific datasheet.