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Description
General description
Purified mouse monoclonal antibody. Recognizes the ~67 kDa VCP protein.
Recognizes the ~67 kDa VCP protein in HePG2 cells and human placenta tissue.
This Anti-VCP Mouse mAb (4A8) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of VCP.
Immunogen
Full-length, recombinant human VCP (aa 1-589), expressed as a GST fusion protein
Application
ELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Physical form
In PBS, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Warning
Toxicity: Regulatory Review (Z)
Analysis Note
Positive Control
HePG2 cells, human placenta tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
- Related Categories Alphabetical Index, Antibodies, Primary Antibodies, V clone
