Description_x000D_ General description_x000D_ Vacuolar protein sorting-associated protein 28 homolog (UniProt: Q9UK41; also known as H-Vps28, ESCRT-I complex subunit VPS28) is encoded by the VPS28 gene (Gene ID: 51160) in human. VPS28 is a component of the Endosomal Sorting Complex Required for Transport (ESCRT)-I complex that regulates the vesicular trafficking process. This complex forms the machinery driving protein sorting from endosomes to lysosomes. It is located in the cell membrane and late endosome membrane. Two isoforms of VPS28 have been described that are generated by alternative splicing. ESCRT complexes play a vital role in receptor down-regulation, lysosome biogenesis, and budding of HIV. In humans, ESCRT-I complex consists of TSG101, VPS28, and one of four potential human VPS37 homologues. The main role of ESCRT-I is to recognize ubiquitinated cargo via the UEV domain of the VPS23/TSG101 subunit. The N-terminal domain of VPS28 contains a conformationally dynamic hydrophobic binding site on its surface. The C-terminal domain of VPS28 is reported to bind the ESCRT-II complex. (Ref.: Kostelansky, MS et al. (2006). Cell 125(1); 113-126)._x000D_ Specificity_x000D_ This rabbit polyclonal antibody detects ESCRT-I complex subunit VPS28 in human cells._x000D_ Immunogen_x000D_ A full-length recombinant ESCRT-I complex subunit VPS28 (VPS28)._x000D_ Application_x000D_ Anti-VPS28, Cat. No. ABS1679, is a rabbit polyclonal antibody that detects ESCRT-I complex subunit VPS28 and has been tested for use in Immunofluorescence and Western Blotting._x000D_ Research Category_x000D_ Signaling_x000D_ Western Blotting Analysis: A representative lot detected recombinant VPS28 protein and VPS28 in cell extracts (Stuchell, M.D., et. al. (2004). J Biol Chem. 279(34):36059-71). Immunofluorescence Analysis: A representative lot detected VPS28 in HEK293T cells transfected with VPS28 (Silvestri, L.S., et. al. (2007). J Infect Dis. 196 Suppl 2:S264-70)._x000D_ Target description_x000D_ ~51 kDa observed; 25.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s)._x000D_ Physical form_x000D_ Affinity Purified_x000D_ Format: Purified_x000D_ Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide._x000D_ Storage and Stability_x000D_ Stable for 1 year at 2-8°C from date of receipt._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Quality_x000D_ Evaluated by Western Blotting in GST-VPS28 recombinant protein._x000D_ Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant GST-VPS28 protein._x000D_ Other Notes_x000D_ Concentration: Please refer to lot specific datasheet.