Description_x000D_ General description_x000D_ Wee1-like protein kinase (UniProt: P30291; also known as EC: 2.7.10.2, WEE1hu, Wee1A kinase) is encoded by the WEE1 gene (Gene ID: 7465) in human. Wee1 is a magnesium-dependent nuclear kinase that belongs to the Serine/Threonine family of protein kinases. It serves as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed Cdk1 prior to the onset of mitosis. Two isoforms of Wee1 have been described that are produced by alternative splicing. In isoform 1 the nucleotide binding site is localized to amino acids 305-313 and its protein kinase domain lies within amino acids 299-569. Wee1 specifically phosphorylates and inactivates cyclin B1-complexed Cdk1, reaching a maximum during G2 phase and a minimum as cells enter into M phase. Phosphorylation of cyclin B1-Cdk1 is shown to occur exclusively on Tyrosine 15. Wee1 levels are believed to increase during S and G2 phases due to increase in transcription and a decline in M phase has been attributed to reduced synthesis and higher degradation. Phosphorylation at serine 642 by BRSK1 and BRSK2 in post-mitotic neurons is reported to down-regulate Wee1 activity in polarized neurons. Also, phosphorylation at serine 53 by PLK1 and at serine 123 by Cdk1 generates a signal for its ubiquitination and proteasomal degradation at the onset of G2/M phase._x000D_ Specificity_x000D_ This rabbit polyclonal antibody detects Wee1-like kinase in human cells. It targets an epitope within 15 amino acids from the C-terminal region._x000D_ Immunogen_x000D_ KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Wee1-like protein kinase. The immunogen sequence is conserved between isoforms 1 and 2._x000D_ Application_x000D_ Anti-Wee1, Cat. No. ABE1336-100UG, is a highly specific rabbit polyclonal antibody that targets Wee1-like protein kinase and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting._x000D_ Research Category_x000D_ Epigenetics & Nuclear Function_x000D_ Western Blotting Analysis: 2 µg/mL from a representative lot detected Wee1 in 10 µg of K562 cell lysate._x000D_ Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Wee1 in human placenta and human testis cancer tissues._x000D_ Target description_x000D_ ~72 kDa observed; 71.60 kDa calculated. Uncharacterized bands may be observed in some lysate(s)._x000D_ Physical form_x000D_ Affinity Purified_x000D_ Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide._x000D_ Storage and Stability_x000D_ Stable for 1 year at 2-8°C from date of receipt._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Quality_x000D_ Evaluated by Western Blotting in HepG2 cell lysate._x000D_ Western Blotting Analysis: 2 µg/mL of this antibody detected Wee1 in 10 µg of HepG2 cell lysate._x000D_ Other Notes_x000D_ Concentration: Please refer to lot specific datasheet.