ANTI-ZDHHC6

Description_x000D_ General description_x000D_ The gene ZDHHC6 (zinc finger DHHC-type containing 6) encodes a transmembrane enzyme containing a conserved cytosolic DHHC domain and is expressed mainly in the brain, uterus, kidney, lung, colon, thymus and small intestine. The gene is mapped to human chromosome 10q25.2 and contains nine exons._x000D_ Immunogen_x000D_ Palmitoyltransferase ZDHHC6, probable recombinant protein epitope signature tag (PrEST)_x000D_ Application_x000D_ All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry._x000D_ The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige._x000D_ Physical form_x000D_ Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide_x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Legal Information_x000D_ Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ Biochem/physiol Actions_x000D_ The gene ZDHHC6 (zinc finger DHHC-type containing 6), also referred to as DHHC-6, encodes a palmitoyltransferase that catalyzes the palmitoylation of calnexin on two juxtamembranous cysteines. It is localized to the ER (endoplasmic reticulum) and targets calnexin to the central ER. It is the only palmitoyltransferase involved in this process out of the 16 DHHC enzymes present in the mammalian ER. Overexpression of DHHC6 leads to increased palmitoylation of endogenous as well as ectopically expressed calnexin. Knock down of DHHC6 results in a change in cell morphology. Palmitoylation of calnexin controls the association of calnexin with the ribosome–translocon complex (RTC) forming a supercomplex. The formation of this supercomplex is necessary for the capture of proteins by chaperones outside the translocon and to initiate folding._x000D_ Features and Benefits_x000D_ Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section._x000D_ Every Prestige Antibody is tested in the following ways:_x000D_ • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues._x000D_ • Protein array of 364 human recombinant protein fragments._x000D_ • Validated for multiple commonly used applications such as IHC (Immunohistochemistry), IF (Immunofluorescence), and WB (Western Blot)_x000D_ Linkage_x000D_ Corresponding Antigen APREST73147.