Description_x000D_ General description_x000D_ Autophagy is an evolutionarily conserved degradation process that targets long-lived proteins, organelles, and other cytoplasmic components for degradation via the lysosomal pathway. Activation of the autophagy pathway is required for multiple cellular roles, including survival during starvation, the clearance of intracellular components, development, and immunity._x000D_ Application_x000D_ Autophagy Assay Kit has been used to determine the formation of autophagosomes by flow cytometry._x000D_ Suitability_x000D_ This kit is suitable for the detection of autophagy in mammalian cells by flow cytometry, fluorescence microscopy, or fluorescence microplate readers._x000D_ Principle_x000D_ The Autophagy Assay kit provides a simple and direct procedure for measuring autophagy in a variety of cell types using a proprietary fluorescent autophagosome marker (λex = 333/λem = 518 nm).