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Description_x000D_
General description_x000D_
Albumin is the most abundant plasma protein in humans. It accounts for ∼60% of the total serum protein. Albumin plays important physiological roles, including maintenance of colloid osmotic pressure and binding of key substances such as long-chain fatty acids, bile acids, bilirubin, hematin, calcium, and magnesium. It has antioxidant and anticoagulant effects, acts as a carrier for nutritional factors and drugs, and is an effective plasma pH buffer. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephritic syndrome, malnutrition, and protein-losing enteropathies. High levels are associated with dehydration._x000D_
Features and Benefits_x000D_
Compatible with high-throughput handling systems. Can be adapted for use with cuvettes._x000D_
Suitability_x000D_
Suitable for the detection of hemoglobin in serum, plasma, urine, and other biological samples._x000D_
Principle_x000D_
The BCG (Bromocresol Green) albumin assay kit is designed to measure albumin directly without any pretreatment of samples. The optimized formulation substantially reduces interference by other substances (lipids/other proteins) in the raw samples. It may also be used to measure effects of drugs and other compounds on albumin metabolism. The procedure involves addition of a single working reagent and a 5 minute incubation. The optimized formulation has greatly enhanced reagent and signal stability. The kit utilizes bromocresol green, which forms a colored complex specifically with albumin. The intensity of the color, measured at 620 nm, is directly proportional to the albumin concentration in the sample.
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