Description; Application; For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins.[1] Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli[2] or the Weber and Osborn[3] SDS-PAGE procedures.; Components; This product contains: • α2-macroglobulin (equine serum) • β-galactosidase (E. coli) • phosphorylase b (rabbit muscle) • catalase (bovine liver) • alcohol dehydrogenase (yeast) • carbonic anhydrase (bovine erythrocyte) • trypsin inhibitor (soy bean) • lysozyme (chicken egg) • aprotinin (bovine lung) Each vial contains approximately 33% protein, 33% NaCl and 33% sucrose.; Other Notes; Contains nine biotinylated proteins (approx 0.1 mg total).; Reconstitution; Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C.