Description_x000D_ General description_x000D_ Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition._x000D_ Application_x000D_ Bromophenol blue sodium salt is used as tracking dye in electrophoresis. It was used in the preparation of protein samples for western blotting analysis. The dye was used in Drosphila fly food to aid visualization of gut for dissection._x000D_ A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels._x000D_ Packaging_x000D_ 5, 10, 25 g in glass bottle_x000D_ Reconstitution_x000D_ A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.