Description_x000D_ Application_x000D_ See technical bulletin for detailed protocols_x000D_ Features and Benefits_x000D_ MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells._x000D_ The canine MDR1 (cP-gp) efflux transporter gene has been effectively disrupted in both alleles. There is no expression of the cP-gp. Human BCRP has been transfected into the MDCKII cell line. Validation studies have shown efflux of standard BCRP substrates._x000D_ Quality_x000D_ Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification._x000D_ Legal Information_x000D_ Exhibit 1: ADME/Tox cell lines license_x000D_ MDCKII subclone was originally isolated by Daniel Louvard, Institut Curie, Paris France._x000D_ References: Hansson, G.C.,Simons, K and Van Meer G (1986) EMBO 5: 483-489, Louvard D (1980) PNAS 77; 4132-4136