Description_x000D_ General description_x000D_ Choline chloride is a quaternary ammonium salt and associates with hydrogen bond donors (HBD). It is essential for the vital physiological functions and is used as a nutritional additive. Choline is a source of methyl groups for methyl-transfer reactions._x000D_ Application_x000D_ Choline chloride has been used:_x000D_ • as a component of choline acetyltransferase (ChAT) buffer and for choline uptake in cultured human neuroblastoma (SK-N-SH) cells_x000D_ • for choline standard curve generation for quantifying phospholipase D (PLD) activity_x000D_ • as a component of slice solution for the dissection of entorhinal-hippocampal brain slices_x000D_ Packaging_x000D_ 1 kg in poly drum_x000D_ 100, 500 g in poly bottle_x000D_ Biochem/physiol Actions_x000D_ The enzymatic activities of butyrylcholinesterase (BChE) and paraoxonase 1 (PON1), two serum enzymes synthesized by the liver and related with inflammation, were decreased in a sepsis animal model injected with LPS. Choline chloride administered intravenously at 20 mg/kg body weight prevents the LPS-mediated decreases in the activities of these two enzymes ._x000D_ Substrates_x000D_ Choline acetyltransferase substrate