{{ isErrorSetToBasket === false ? 'Товар добавлен вкорзину' : 'Не удалось добавить товар в корзину'}}
Перейти в корзину
{{Object.keys(error)[0]}}:
{{Object.values(error)[0]}}
COLLAGENASE FROM CLOSTRIDIUM HISTOLYTIC&
Кат. №: C7657-1G
Цена По запросу
Количество
Вы уже добавили максимально доступное на складе кол-во товара
Достигнуто максимально доступное кол-во
Под заказ
{{!!storageProduct ? 'На складе' : 'Под заказ'}}
Ожидается поставка
Description_x000D_
Application_x000D_
Clostridiopeptidase A has been used to study the avoidance of the progression of necrotic tissue in neonates as well as to evaluate the effect of clostridiopeptidase A collagenase (CAC) in a rat model of postoperative peritoneal adhesion. Clostridiopeptidase A has also been used in a study to describe a quantitative method to assess small amounts of collagen based on MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass spectrometry (MS) subsequent to digestion of collagen._x000D_
This product has been used in the isolation of pancreatic ductal adenocarcinoma (PDAC) cell lines and the tissue culture of pancreatic fibroblasts._x000D_
Biochem/physiol Actions_x000D_
Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of the reaction varies from 15 minutes to several hours to yield a satisfactory efficiency of cell dissociation without causing too much cell death. Krebs Ringer buffer with calcium and BSA is preffered and Zn2+ is required for activity. This enzyme is tested for the release of hepatocytes at approximately 1 mg/mL concentration, in a total volume of 100 mL for each rat liver._x000D_
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N',N'-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline._x000D_
Quality_x000D_
Also contains clostripain, nonspecific neutral protease, and tryptic activities._x000D_
Unit Definition_x000D_
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT._x000D_
Preparation Note_x000D_
A stock solution may be prepared by dissolving 0.05-0.1 mg/mL of collagenase in 50 mM TES buffer containing 0.36 mM calcium chloride (TESCA), pH 7.4, at 37 °C.
- Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology, Cell Dissociation, Cell Dissociation and Cell Lysis, Cell Signaling Enzymes, Cell Signaling and Neuroscience, Collagenases, Core Bioreagents, Core Bioreagents Enzymes, Crude Collagenase, Use-Tested, Cytoskeleton and Extracellular Matrix, Enzyme Class Index, Enzymes for Diagnostic Kit Manufacturing, Enzymes, Inhibitors, and Substrates, Extracellular Matrix, Extracellular Matrix Peptides and Proteins, Histology, MMP Enzymes, Matrix Metalloproteinases Products, Proteases, Proteases & Protein Sequencing, Proteolytic Enzymes, Proteolytic Enzymes and Substrates, Research EssentialsMore... Quality Level