CREATININASE FROM PSEUDOMONAS SP.

Description_x000D_ Application_x000D_ Creatininase from microorganisms may be used in the preparation of amperometric biosensor by co-immobilization with other enzymes for the determination of creatinine._x000D_ This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis._x000D_ Packaging_x000D_ 1000 units in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site._x000D_ Physical properties_x000D_ Isoelectric point: 4.7_x000D_ Michaelis constants: 3.2 x 10‾2M (Creatinine), 5.7 x 10‾2M (Creatine)_x000D_ Structure: 6 subunits per mol of enzyme (One mol of zinc is bound to each subunit)_x000D_ Inhibitors: Ag+, Hg++, N-bromosuccinimide, EDTA_x000D_ Optimum pH: 6.5 − 7.5_x000D_ Optimum temp: 70°C_x000D_ pH Stability: pH 7.5 − 9.0 (5°C, 16hr)_x000D_ Thermal stability: Below 70°C (pH 7.5, 30 min)_x000D_ Unit Definition_x000D_ One unit will hydrolyze 1.0 mmole of creatinine to creatine per min at pH 8.0 and 25 °C_x000D_ Physical form_x000D_ Lyophilized powder containing sucrose and BSA as stabilizers_x000D_ Analysis Note_x000D_ Protein determined by biuret.