CRYOPLATEABLE PRIMARY HUMAN HEPATOCYTES

Description_x000D_ General description_x000D_ We provide high viability cryoplatable hepatocytes, sourced from LifeNet Health® that predictably represents in vivo conditions. Applications for primary hepatocytes include in vitro evaluation of metabolism, drug-drug interaction, drug transporter activity, and toxicity of drug candidates. There are many other applications that encompass cryoplatable hepatocytes._x000D_ Application_x000D_ See technical bulletins for detailed protocols._x000D_ Click lot availability link to get a complete list of currently available hepatocyte lots from LifeNet Health®._x000D_ Once the lot number(s) of interest are determined enter the lot number into the “Certificate of Analysis” request search engine at the bottom of the page to obtain a pdf copy of the CoA. Note, lot number CoA upload is only available on the product number detail page corresponding with the lot number._x000D_ Features and Benefits_x000D_ These cryoplatable hepatocytes are sourced from LifeNet Health®. All cryoplatable hepatocytes have post-thaw viabilities over 80%. These lots are also characterized for Phase I CYP450 isoform and Phase II drug-metabolizing enzyme activity. Cryoplatable hepatocytes can be used for a variety of applications including metabolic stability, clearance, metabolite ID, metabolite profiling, species comparison studies, and uptake transporter studies._x000D_ Each lot is fully characterized for viability/stability, cell yield, attachment efficiency, optimal seeding density, monolayer confluency, Phase I and Phase II drug metabolizing enzyme activities and cyp induction. We have a wide selection of lots that can be used for a variety of applications, including, but not limited to:_x000D_ • Drug metabolizing enzyme activity (stability, clearance, species, metabolite profiling and ID)_x000D_ • Time-dependent metabolism (plated assay for low-clearance compounds)_x000D_ • CYP450 Induction (mRNA and activity)_x000D_ • Plated drug transporter assays (uptake and efflux)_x000D_ • Toxicity assays_x000D_ • High content analysis_x000D_ • High content imaging_x000D_ • 3D culture_x000D_ Packaging_x000D_ This product will be shipped in a liquid nitrogen dry shipper supplied from Cryoport. Please remember to ship the container back to Cryoport once you have removed the materials. Additional Cryoport shipping charges may apply._x000D_ Please see the Cryoport website for more details on the dry shipper._x000D_ Legal Information_x000D_ LifeNet Health is a registered trademark of LifeNet Health