Dihydroethidium 1PC X 25MG

Description General description A cell-permeable, chemically reduced ethidium derivative that can be used as a fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, however, following oxidation to ethidium and subsequent intercalation to DNA, a bright red fluorescence is achieved. Often used to determine the level of superoxide anion in cells by flow cytometry. A cell-permeable, chemically-reduced ethidium derivative. Commonly used to analyze respiratory burst in phagocytes. Useful fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, whereas after oxidation of this probe to ethidium, it intercalates cell’s DNA, staining the nucleus with a bright red fluorescence. Packaging 25 mg in Glass bottle Biochem/physiol Actions Cell permeable: yes Primary Target Useful fluorogenic probe for the detection of reactive oxygen species (ROS) Product does not compete with ATP. Reversible: no Packaging Packaged under inert gas Warning Toxicity: Irritant (B) Reconstitution Following reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Other Notes Haugen, T.S., et al. 1999. Eur. Respir. J.14, 1100. Mancini, M., et al. 1997. J. Cell Biol.138, 449. Carter, W.O., et al. 1994. J. Leukoc. Biol.55, 253. Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187. Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117. Perticarari, S., et al. 1991. Cytometry 12, 687. Bucana, C.D., et al. 1990. Exp. Cell Res.190, 69. Rothe, G., and Valet, G. 1990. J. Leukoc. Biol.47, 440.; Description