DULBECCO'S MODIFIED EAGLE'S MEDIUM/NUTRI

Description_x000D_ General description_x000D_ A measured mixture of chemically defined ingredients makes up a basal medium, which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/F-12 is a completely defined synthetic medium, which is protein-free._x000D_ Application_x000D_ DMEM:F12 is a 50:50 mixture of DMEM and Ham’s F12 media that has proven to be useful in a wide range of cell culture applications, especially when supplemented with fetal bovine serum (FBS). Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells._x000D_ Suitable for growth of wide range of cell types in low serum conditions._x000D_ Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is used in cell culture & proliferation assays. It is also used in bone marrow-derived macrophages culture._x000D_ Linkage_x000D_ Need FBS/ We can assist with your serum planning. Please visit the Serum Planner for more information._x000D_ Reconstitution_x000D_ Supplement with 0.365 g/L L-glutamine.