Description_x000D_ General description_x000D_ Duolink™ In Situ PLA® Probe Anti-Mouse MINUS contains affinity purified donkey anti-mouse IgG (H+L), which reacts with whole molecule mouse IgG and also reacts with the light chains of other mouse immunoglobulins._x000D_ Application_x000D_ Duolink™proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples._x000D_ This product can be applied to both the Duolink™ In Situ Fluorescence Protocol and the Duolink™ In Situ Brightfield Protocol depending on the detection reagents used._x000D_ Visit our Duolink™ PLA Resource Center for information on how to run a Duolink™ experiment, applications, troubleshooting, and more._x000D_ To perform a complete Duolink™ PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink™ PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection._x000D_ Specificity_x000D_ PLA probe anti-Mouse reacts with whole molecule Mouse IgG and the light chains of other Mouse immunoglobulin′s. The PLA probe anti-Mouse may cross-react with Rat antibodies, but has minimal cross reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, and sheep serum proteins. A PLUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information._x000D_ Application Note_x000D_ Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink™PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required._x000D_ Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink™ projects_x000D_ View full Duolink™ product list_x000D_ Legal Information_x000D_ Duolink is a trademark of Sigma-Aldrich Co. LLC_x000D_ PLA is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ Features and Benefits_x000D_ • No overexpression or genetic manipulation required_x000D_ • High specificity (fewer false positives)_x000D_ • Single molecule sensitivity due to rolling circle amplification_x000D_ • Relative quantification possible_x000D_ • No special equipment needed_x000D_ • Quicker and simpler than FRET_x000D_ • Increased accuracy compared to co-IP_x000D_ • Publication-ready results_x000D_ Components_x000D_ This product is comprised of the following:_x000D_ • 5x PLA Probe Anti-Mouse MINUS - Donkey anti-mouse secondary antibody conjugated to oligonucleotide MINUS_x000D_ • 1x Blocking Solution - Reagent for blocking of the sample_x000D_ • 1x Antibody Diluent - For dilution of PLA probes and primary antibodies_x000D_ See datasheet for more information._x000D_ Preparation Note_x000D_ Storage and Stability: Store the PLA Probe Anti-Mouse MINUS at 2–8°C. Do not store the diluted PLA Probe Anti-Mouse MINUS solution.