EX-CELL(TM) ACF CHO MEDIUM

Description_x000D_ Features and Benefits_x000D_ Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. It also supports high cell densities and maintenance of these densities of viable cells for extended periods resulting in increased productivity._x000D_ Other Notes_x000D_ Proprietary formulation containing inorganic salts, HEPES, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants._x000D_ Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components._x000D_ Animal component-free medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells._x000D_ Reconstitution_x000D_ Powdered media are extremely hygroscopic and should be protected from atmospheric moisture. Preparing a concentrated solution of medium is not recommended as precipitates may form._x000D_ 1. Measure out 90% of final required volume of tissue culture grade water. Water temperature should be 25-40oC._x000D_ 2. While stirring the water, add the powdered medium (21.5 g/L). Heat may enhance solubility but do not go above 40oC._x000D_ 3. Rinse original package with a small amount of water to remove all traces of the powder. Add to solution in step 2._x000D_ 4. Stir until dissolved._x000D_ 5. Add 0.6g/L (range 0.6-1.2g/L or higher depending on individual cell line) of G3126 L-Glutamine._x000D_ 6. Add 1.5 g/L of S8875 sodium bicarbonate (If increased buffering capacity is desired this amount can be increased to 3.0 g/L)._x000D_ 7. Adjust the pH to 7.45 range (7.3-7.6) using 5 N NaOH._x000D_ 8. Use additional water to bring the solution to final volume._x000D_ 9. Sterilize immediately by filtration using a membrane with a porosity of <0.22 microns. Do not use poly-ether sulfones (PES) type of membranes. We recommend PVDF as the best inert type of filter membrane._x000D_ 10. Aseptically dispense medium into a sterile container. Store at 2-8 °C in the dark._x000D_ Legal Information_x000D_ EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC