FURA 2/AM 1PC X 1SET

Description General description Cell permeant ester form of FURA 2 (Cat. No. 344900). The active dye is rapidly released in the cell by cytoplasmic esterases. Fluorescent calcium chelator (Kd = 224 nM) that offers 30 times greater fluorescence intensity than QUIN 2 (Cat. No. 551826). Can be used as a probe in many cellular systems, including cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max.: 362 nm, emission max.: 512 nm) differ from those during high calcium conditions (excitation max.: 335 nm, emission max.: 505 nm). Note: 1 set = 10 x 100 µg. Cell-permeable ester form of FURA 2. Note: 1 set = 10 x 100 µg. PLURONIC® F-127, PROTEIN GRADE Detergent (Cat. No. 540025, available separately) is a mild, low-toxicity detergent that can aid in the solubilization of FURA 2/AM. Packaging 1 mg in Plastic ampoule 1 set in Glass bottle Biochem/physiol Actions Cell permeable: yes Primary Target Ratiometric calcium indicator Product does not compete with ATP. Reversible: no Packaging Packaged under inert gas Warning Toxicity: Standard Handling (A) Preparation Note Further dilute with aqueous buffers just prior to use. Reconstitution Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 1 month at -20°C. Other Notes Davis, M.J., et al. 1992. Am. J. Physiol. 263, H1292. Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol.47, 226. Schwartz, Y., et al. 1992. Endocrinology131, 772. Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937. Janconi, M.E., et al. 1990. J. Cell Biol.110, 1555. Tsien, R.Y. 1980. Biochemistry19, 2396. Legal Information Pluronic is a registered trademark of BASF; Description