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G 418 DISULFATE SALT BIOREAGENT, SUITAB&
Кат. №: A1720
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Description
General description
Chemical structure: aminoglycoside
G-418 is an aminoglycoside antibiotic, which is produced by Micromonospora rhodorangea NRRL 5326 strain. It has a broad range of antibacterial activity both in vivo and in vitro. It also shows activity against protozoa, cecal amoebiasis, and helminthes. It is commonly known as geneticin selective antibiotic.
Application
G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.
Packaging
1, 5, 25 g in glass bottle
Biochem/physiol Actions
G-418 inhibits protein synthesis at the early stages of elongation, post-initiation, at the initiation stage of translation as well as just before the initiation stage of translation.
Caution
The product is stable for three years when stored at 2-8°C. Stock solutions are stable at 37°C for 8 days.
Preparation Note
G418 powder is soluble in water at 50 mg/mL.
Other Notes
Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.
- Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotics, Antibiotics A to Z, Antibiotics G-M, Antibiotics by Application, Antibiotics by Mechanism of Action, Antineoplastic and Immunosuppressive Antibiotics, Biochemicals and Reagents, Cell Culture, Cell Culture Antibiotics as Powder, Chemical Structure Class, Core Bioreagents, Genetic Marker Selection, Interferes with Protein Synthesis, Life Science Reagents for Cell Culture, Life Science Reagents for Cloning, Life Science Reagents for Transfection, Reagents and Supplements, Research Essentials, Spectrum of Activity More... Quality Level