GENELUTE PCR CLEAN-UP KIT

Description_x000D_ General description_x000D_ The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform._x000D_ Application_x000D_ GenElute™ PCR Clean-Up Kit has been used for rapid purification of single-stranded or double-stranded PCR amplification products (100bp to 10kb) from other components in the reaction._x000D_ Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis._x000D_ Features and Benefits_x000D_ • Purifies up to 100 μl or 10 μg of PCR amplified DNA in 8 minutes_x000D_ • Recovers up to 95% of PCR products between 100 bp and 10 kb_x000D_ • Removes over 99% of primers and other components_x000D_ • Eliminates the need to remove mineral oil by organic extraction_x000D_ • 40% more purification preps supplied than market leader_x000D_ Other Notes_x000D_ The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform._x000D_ Principle_x000D_ The GenElute PCR Clean-Up Kit combines the advantages of silica binding with a microspin format. The DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp are removed)._x000D_ Legal Information_x000D_ GenElute is a trademark of Sigma-Aldrich Co. LLC