Description_x000D_ General description_x000D_ Chemical structure: aminoglycoside_x000D_ Gentamicin, also known as gentiomycin C, is an aminoglycoside, which is naturally produced by Gram-negative bacteria. This antibiotic functions by preventing protein synthesis. Gentamicin sulfate prevents the growth of both Gram positive and Gram negative bacteria, in vivo and in vitro. In tissue culture, gentamicin sulfate is used to inhibit the growth of various strains of mycoplasma._x000D_ Application_x000D_ Gentamicin sulfate is a broad-spectrum antibiotic used as a selection agent (gentamicin-resistance gene) in molecular biology and cell culture applications. This product is formulated to contain 50 mg/mL Gentamicin in deionized water and is recommended for use in eukaryotic cell culture at a volume of 1 milliliter per liter._x000D_ Gentamicin sulfate solution has been used as a component of different types of culture media._x000D_ Biochem/physiol Actions_x000D_ Gentamicin is recommended for treating sepsis during emergency treatment. It is an alternate to ceftriaxone for treating genital infection._x000D_ Mode of Action: Gentamicin inhibits protein synthesis by binding to the 30S subunit of the ribosome._x000D_ Antimicrobial spectrum: Includes Gram-negative and Gram-positive bacteria, including strains resistant to tetracycline, chloramphenicol, kanamycin and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus and Streptococcus._x000D_ Components_x000D_ Gentamicin is an aminoglycoside complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is used as the sulfate salt. There are three components, each consisting of five basic nitrogens and requiresing five equivalents of sulfuric acid per mole of gentamicin base._x000D_ Caution_x000D_ The product is stable at 37° for 5 days and is stored at 2-8°C.