GLYCEROL DEHYDROGENASE FROM CELLULOMONA&

Description_x000D_ General description_x000D_ Glycerol Dehydrogenase belongs to iron-containing alcohol dehydrogenase family. It is made up of eight identical subunits which are about 42,000 Da each. The C-terminal has histidine and the N-terminal has serine amino acid._x000D_ Application_x000D_ Glycerol Dehydrogenase (GDH) from Cellulomonas sp. has been used in the determination of glycerol by sequential injection analysis (SIA) method. It has also been used in the preparation of pyrroloquinoline quinone (PQQ) based biosensor for the determination of glycerol._x000D_ This enzyme is useful for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase in clinical analysis. Formation of NADH from the reaction of glycerol and NAD+ was catalyzed by the enzyme glycerol dehydrogenase._x000D_ Packaging_x000D_ 250, 1000 units in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Glycerol dehydrogenase catalyzes the conversion of glycerol to glycerone._x000D_ Physical properties_x000D_ Isoelectric point : 4.4 ± 0.1_x000D_ Michaelis constants : 1.1 x 10¯2M (Glycerol), 8.9 x 10¯5M (NAD+)_x000D_ Structure : 10 subunits (42,000) per mol of enzyme_x000D_ Inhibitors : p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal_x000D_ ions (Co++, Ni++, Cu++, Zn++, Cd++)_x000D_ Optimum pH : 10.0 – 10.5_x000D_ Optimum temperature : 50°C_x000D_ pH Stability : pH 7.5 – 10.5 (25°C, 20hr)_x000D_ Thermal stability : below 55°C (pH 7.5, 15min)_x000D_ Substrate specificity : This enzyme has the highest specificity for glycerol and 1,2-propanediol, and also oxidizes glycerol-α-monochlorohydrin, ethylene_x000D_ glycol and 2,3-butanediol. The oxidative reaction is stimulated by K+, NH+4 and Rb+._x000D_ Unit Definition_x000D_ One unit will oxidize 1.0 μmole of glycerol to dihydroxyacetone per min at 25 °C at pH 10.0._x000D_ Physical form_x000D_ Lyophilized powder containing bovine serum albumin