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Description_x000D_
General description_x000D_
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders._x000D_
Goat Anti-Human IgG (Fc Specific)-Agarose antibody is specific for the Fc fragment of human IgG when tested against purified human IgA, IgG, IgM, Fc, and kappa and lambda light chains. No reactivity with mouse or rat IgG is seen by Ouchterlony Double Diffusion (ODD), prior to agarose bead coupling._x000D_
Immunogen_x000D_
Fc fragment of human IgG._x000D_
Application_x000D_
Anti-Human IgG (Fc Specific)-Agarose antibody produced in goat has also been used to purify galanin 1 (Gala1), 3Gal-substituted P-selectin glycoprotein ligand-1 (PSGL-1)/hIgG1 from supernatants of transfected COS cells._x000D_
Goat Anti-Human IgG (Fc Specific)-Agarose antibody is suitable for use in Ouchterlony double diffusion. The antibody has also been used for affinity purification assays._x000D_
Immunoprecipitation assays were performed using goat anti-human IgG (Fc specific) crosslinked to agarose beads. The beads were diluted in 1 ml of 5% FBS/DMEM for 2 hours at 4 degrees._x000D_
Physical form_x000D_
Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide_x000D_
Disclaimer_x000D_
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_
Other Notes_x000D_
Antibody adsorbed with mouse and rat IgG
- Related Categories Agarose, Alphabetical Index, Antibodies, Antibody Purification and Characterization, Antibody-Agaroses, HU-HZ, Human IgG Secondary Antibodies and Conjugates, Primary Antibodies, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies, Supplementary ProductsMore... conjugate