Description_x000D_ General description_x000D_ IgG antibody plays a crucial role in humoral immune responses such as phagocytosis, complement activation, placental transport and cell surface-receptor binding. Anti-human IgG (Fc specific), F(ab’)2 fragment antibody can be used as a capture antibody in antibody-binding assay for soluble ICAM-1-Fc. Goat anti-human-IgG (Fc specific) F(ab’)2 antibody reacts specifically with human IgG but does not react with other immunoglobulins._x000D_ IgG is the main type of antibody in human serum, secreted by the B cells. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are linked by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4._x000D_ Immunogen_x000D_ Purified human IgG_x000D_ Application_x000D_ Anti-Human IgG (Fc specific), F(ab′)2 fragment antibody produced in goat has been used in studying the antibody binding assay of soluble intercellular adhesion molecule-1 (ICAM-1)-Fc._x000D_ Anti-human IgG (Fc specific), F(ab’)2 fragment antibody can be used in ELISA to determine human IgG1-Fc protein._x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% sodium azide_x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ IgG stimulates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a crucial role in antibody-dependent cell-mediated cytotoxicity (ADCC). IgG might be associated with allergy. It has the longest serum half life._x000D_ Other Notes_x000D_ Antibody adsorbed with mouse and rat IgG_x000D_ Preparation Note_x000D_ Adsorbed to reduce background with mouse or rat samples._x000D_ Useful when trying to avoid background staining due to the presence of Fc receptors.