Description_x000D_ Application_x000D_ Grace′s medium was originally formulated to support the growth of cells derived from the Australian emperor gum moth, Antherea eucalypti. The medium is a modification of Wyatt′s medium to more closely resemble Antherea hemolymph. The cell lines established by Grace using this medium were the first continuous lines developed. The basal medium, when properly supplemented, has been used to culture cells derived from a variety of insects including several species of lepidopterans as well as some dipterans. The medium is primarily used as a basal medium for the growth and maintenance of cell lines derived from lepidopterans.