Description_x000D_ General description_x000D_ Lipoproteins transport the majority of plasma lipids including cholesterol and triglycerides. High-density lipoprotein (HDL), low-density lipoprotein (LDL), and very-low-density lipoprotein (VLDL) are the lipoproteins responsible for the vast majority of cholesterol transport in the blood. High LDL levels and low HDL levels are strongly associated with increased risk of adverse cardiovascular events._x000D_ Application_x000D_ HDL and LDL/VLDL Quantitation Kit has been used to determine the concentrations of HDL and LDL/VLDL in serum samples._x000D_ Suitability_x000D_ Suitable for use with plasma and serum samples._x000D_ Principle_x000D_ In this kit, serum HDL and LDL/VLDL are first separated and then the cholesterol concentration of each is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the cholesterol present. This kit can also be used to determine the concentration of free cholesterol and cholesteryl esters present in a sample.