HISTONE DEACETYLASE 3 (HDAC3) ACTIVITY&

Description_x000D_ General description_x000D_ Histone deacetylases (HDACs) are a large family of enzymes that remove acetyl groups from histone proteins. Site specific histone acetylation and deacetylation have been shown to activate or repress eukaryotic gene transcription, respectively, and as a consequence, it plays a crucial role in mammalian development and disease. HDACs are involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence._x000D_ With Sigma′s HDAC3 Activity Assay Kit, HDAC3 present in a test sample will act with the supplied Developer, to deacetylate and then cleave the HDAC3 Substrate [R-H-K-K(Ac)-AFC]. This activity will release the quenched fluorescent group, AFC, which can be detected at Em/Ex = 380/500 nm. Trichostatin A is an HDAC inhibitor included in the kit to verify HDAC3 activity. The kit provides a rapid, simple, sensitive and reliable test. It is suitable for either individual tests or high throughput assays, from nuclear extracts, purified, or immunoprecipitated HDAC3, and from native, recombinant, or genetically modified HDAC3._x000D_ Features and Benefits_x000D_ • Simple, sensitive, and reliable assay_x000D_ • Simple procedure; takes ~60 min_x000D_ • Utilizes fluorometric methods_x000D_ • Sample type: cell and tissue lysates, plasma and serum, other biological fluids_x000D_ • Species reactivity: mammalian_x000D_ • Suitable for individual tests or high throughput assays and kinetic studies_x000D_ • Convenient 96-well microplate format_x000D_ • Suitable for high throughput measurement of HDAC3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC3 samples