Description_x000D_ Application_x000D_ Hyaluronidase is an enzyme derived from bovine testes. It is used to remove the cumulus mass from newly ovulated eggs, when preparing the eggs for in vitro culture._x000D_ Reconstitute 1 vial of hyaluronidase with 3 ml of M2 Medium (Product Nos. M 7167 or M 5910) or deionized water to obtain a 10 mg/ml stock solution. Dilute stock solution with M2 Medium to obtain a final working concentration of approximately 300 μg/ml._x000D_ Gonadotropins are used to superovulate 20 female mice. The mice are then mated and preimplantation stage embryos are retrieved and counted. The embryos are treated with hyaluronidase until the cumulus mass is dissociated from the embryos. The embryos are cultured, viewed daily and progress of the embryos is recorded._x000D_ Biochem/physiol Actions_x000D_ Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression._x000D_ These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates._x000D_ Unit Definition_x000D_ One unit will cause a change in % transmittance at 600 nm of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 mL reaction mixture (45 minute assay).