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Description_x000D_
General description_x000D_
A Protein Conformational Array (PCA) in a sandwich ELISA format where the plate is coated with a panel of antibodies raised against peptides derived from the full length protein sequence of seven commercial monoclonal antibody biologic drugs (Biologics). Taken individually, each of these antibodies is strongly antigenic to the peptide sequence that was used in its production. However, when these peptides are incorporated into a full length correctly folded protein, the antigenicity of many of them is masked by the three dimensional structure of the protein and only a limited number of the antibodies respond. The result is a histogram which can be likened to a ‘fingerprint’ for any given monoclonal antibody. Once that ‘fingerprint’ is established, then any changes that occur in that mAb during purification or other manipulations will likely expose a new antigenic site which can be detected by the antibodies on the plate._x000D_
Application_x000D_
For research use only. Not for use in diagnostic procedures.
- Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs antibody form