Description General description A highly selective cell-permeable protein kinase C (PKC) inhibitor (Ki = 10 nM) that is structurally similar to staurosporine. Acts as a competitive inhibitor for the ATP-binding site of PKC. Shows high selectivity for PKCα, βI, βII, γ, δ, and ε isozymes. May inhibit protein kinase A at a much higher concentration (Ki = 2 µM). Also inhibits telomerase A treated NPC-076 cells. A 1 mg/ml solution of Bisindolylmaleimide I (Cat. No. 203290) in anhydrous DMSO. Packaging 1 ml in Glass bottle Biochem/physiol Actions Cell permeable: yes Primary Target PKC Product competes with ATP. Reversible: yes Target Ki: 10 nM against PKC Packaging Packaged under inert gas Warning Toxicity: Irritant (B) Physical form A 1 mg/ml solution of Bisindolylmaleimide I (Cat. No. 203290) in anhydrous DMSO. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Other Notes Ku, W.-C., et al. 1997. Biochem. Biophys. Res. Commun.241, 730. Gekeler, V., et al. 1996. Br. J. Cancer74, 897. Kiss, Z., et al. 1995. Biochim. Biophys. Acta1265, 93. Muid, R.E., et al. 1991. FEBS Lett.293, 160. Toullec, D., et al. 1991. J. Biol. Chem.266, 15771.