Description_x000D_ General description_x000D_ Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration._x000D_ Application_x000D_ Intracellular hydrogen peroxide assay kit has been used to measure intracellular hydrogen peroxide levels._x000D_ Features and Benefits_x000D_ Compatible with high-throughput handling systems._x000D_ Suitability_x000D_ This kit is suitable for the detection of hydrogen peroxide in living cells and a variety of samples such as cell extracts and liquids._x000D_ Principle_x000D_ The Intracellular Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in living cells and in a variety of other samples such as cellular extracts. This kit utilizes a unique cell-permeable sensor that generates a fluorescent product (λex = 490/λem = 520 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader, flow cytometer, or fluorescent microscope.