Description_x000D_ General description_x000D_ KiCqStart Probe qPCR ReadyMix is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product._x000D_ A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. KiCqStart Probe qPCR ReadyMix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification._x000D_ Application_x000D_ KiCqStart® Probe qPCR ReadyMix™ has been used for gene expression analysis by qPCR._x000D_ Features and Benefits_x000D_ • Assay results in as little as 33 minutes_x000D_ • Highly efficient and sensitive real-time PCR results_x000D_ • Little/no optimization required_x000D_ Components_x000D_ 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, insert blue qPCR dye, and stabilizers._x000D_ packaging:_x000D_ 250 reactions* = 2 X 1.25 mL tubes_x000D_ 1250 reactions* = 10 X 1.25 mL tubes_x000D_ 5000 reactions* = 1 X 50 mL tube_x000D_ *number of reactions based on a 20uL volume_x000D_ Legal Information_x000D_ Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc._x000D_ CFX384 is a trademark of Bio-Rad Laboratories, Inc._x000D_ CFX96 is a trademark of Bio-Rad Laboratories, Inc._x000D_ Chromo4 is a trademark of Bio-Rad Laboratories, Inc._x000D_ KiCqStart is a registered trademark of Qiagen Beverly Inc._x000D_ LightCycler is a registered trademark of Roche_x000D_ Mastercycler is a registered trademark of Eppendorf AG_x000D_ MiniOpticon is a trademark of Bio-Rad Laboratories, Inc._x000D_ Opticon is a trademark of Bio-Rad Laboratories, Inc._x000D_ ReadyMix is a trademark of Sigma-Aldrich Co. LLC_x000D_ Rotor-Gene is a registered trademark of Qiagen GmbH_x000D_ Scorpions is a registered trademark of Qiagen Group_x000D_ SmartCycler is a registered trademark of Cepheid, Inc._x000D_ TaqMan is a registered trademark of Roche Molecular Systems, Inc.