Description_x000D_ General description_x000D_ KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR._x000D_ Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. The optimized formulation includes SYBR Green I dye, an antibody-mediated Hot-Start Taq DNA polymerase, dNPTs, MgCl2 and proprietary buffers and stabilizers._x000D_ Application_x000D_ KiCqStart® SYBR® Green qPCR ReadyMix™ has been used for the amplification and quantification of DNA in real-time PCR (qPCR) assay. It has also been used in the amplification and quantification of transcripts in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR)._x000D_ PCR applications:_x000D_ • Gene expression_x000D_ • DNA quantitation_x000D_ • CHiP_x000D_ Features and Benefits_x000D_ • Assay results in as little as 33 minutes_x000D_ • Highly efficient and sensitive real-time PCR results_x000D_ • Little/no optimization required_x000D_ Components_x000D_ 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, ROX Reference Dye (for 580-585 nm excitation), and stabilizers_x000D_ packaging:_x000D_ 250 reactions* = 2 X 1.25 mL tubes_x000D_ 1250 reactions* = 10 X 1.25 mL tubes_x000D_ 5000 reactions* = 1 X 50 mL tube_x000D_ *number of reactions based on a 20uL volume_x000D_ Quality_x000D_ Kit components are free of contaminating DNase and RNase. KiCqStart® SYBR® Green qPCR ReadyMix™, Low ROX is functionally tested in qPCR. Kinetic analysis must demonstrate linear resolution over six orders of dynamic range (r2 > 0.995) and a PCR efficiency > 90%._x000D_ Other Notes_x000D_ Storage Conditions:_x000D_ KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C._x000D_ Legal Information_x000D_ 6538 is a trademark of American Type Culture Collection_x000D_ Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries_x000D_ KiCqStart is a registered trademark of Qiagen Beverly Inc._x000D_ Mx3000P is a registered trademark of Agilent Technologies, Inc._x000D_ Mx3005P is a registered trademark of Agilent Technologies, Inc._x000D_ Mx4000 is a trademark of Agilent Technologies, Inc._x000D_ ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries_x000D_ ReadyMix is a trademark of Sigma-Aldrich Co. LLC_x000D_ Rotor-Gene is a registered trademark of Qiagen GmbH_x000D_ SYBR is a registered trademark of Life Technologies