L-LACTIC DEHYDROGENASE FROM RABBIT&

Description_x000D_ General description_x000D_ Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate._x000D_ Isoelectric point: 8.4-8.6_x000D_ Optimal pH : 7.5 ._x000D_ Packaging_x000D_ 1000, 5000 units in glass bottle_x000D_ 25000, 50000, 100000 units in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids._x000D_ Unit Definition_x000D_ One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C._x000D_ Analysis Note_x000D_ Protein determined by biuret.