Description_x000D_ General description_x000D_ L(+)-Lactate is a metabolic compound formed in animals by the action of the enzyme Lactate Dehydrogenase. Lactate is produced in proliferating cells and during anaerobic conditions such as exercise. Abnormally high concentrations of lactate have been related to pathological conditions such as cancer, diabetes, and lactate acidosis. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood at levels of around 1-2 mmol/L. This kit provides a convenient means for detecting L(+)-Lactate in biological samples._x000D_ Application_x000D_ Lactate Assay Kit II has been used to measure the levels of lactate in samples._x000D_ Suitability_x000D_ Suitable for detecting L(+)-Lactate in culture medium, fermentation medium, blood and cells._x000D_ Principle_x000D_ Lactate concentration is determined by an enzyme assay, which results in a colorimetric (450 nm) product, proportional t the lactate present. There is no need of pretreatment or purification of samples. Typical sensitivities of detection are between 0.02-10 mM of lactate.