Description_x000D_ General description_x000D_ Lectin from Galanthus nivalis (snowdrop) is a tetrameric, 12 kDa mannose binding lectin. It belongs to the family, Amaryllidacea. It is specific to α-1,3- or 1,6- linked D-mannose of carbohydrates. Snowdrop lectins are toxic to insects._x000D_ Application_x000D_ Lectin from Galanthus nivalis (snowdrop) has been used:_x000D_ • to coat ELISA plates_x000D_ • as reverse transcriptase inhibitor_x000D_ • in ELISA for detection of envelope glycoproteins of the human and simian immunodeficiency viruses_x000D_ Packaging_x000D_ 5 mg in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Lectin from Galanthus nivalis (snowdrop) provide resistance against pests and are used in transgenic plants. Snowdrop lectin functions as a carrier to transport fused proteins to the hemolymph as it crosses gut epithelium of target species to exhibit insecticidal effects._x000D_ GNL agglutinates rabbit erythrocytes; human erythrocytes are not agglutinated. GNL sugar specificity is directed to the nonreducing end of the terminal α-D-mannosyl residue of glycoconjugates._x000D_ Preparation Note_x000D_ Purified by affinity chromatography according to Kaku._x000D_ Analysis Note_x000D_ Agglutination activity is expressed in μg/ml and is determined from serial dilution in phosphate buffered saline, pH 7.2 of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of rabbit erythrocytes after 1 hr incubation at 25 °C.