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LYSOZYME FROM CHICKEN EGG WHITE
Кат. №: L6876-25G
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Description_x000D_
Application_x000D_
Lysozyme from chicken egg white has been used for the extraction of genomic DNA from bacterial cells. It has been used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis._x000D_
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts._x000D_
Packaging_x000D_
1, 5, 10, 25, 100 g in poly bottle_x000D_
Biochem/physiol Actions_x000D_
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane._x000D_
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M)._x000D_
Unit Definition_x000D_
One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path)._x000D_
Other Notes_x000D_
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
- Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Bacterial, Biochemicals and Reagents, Carbohydrate Hydrolysis, Carbohydrate and PTM Analysis, Carbohydrate hydrolysis & PTM analysis, Cell Biology, Cell Dissociation and Cell Lysis, Cell Lysis Reagents, Cell Lysis Reagents Enzymes, Cell Signaling and Neuroscience, Core Bioreagents, Core Bioreagents Enzymes, Enzymatic Cell Lysis and Protoplast Preparation, Enzyme Class Index, Enzymes, Inhibitors, and Substrates, Lysing Enzymes and Protoplast Preparation, Research Essentials, Signal Transduction Research ToolsMore... Quality Level