Description_x000D_ General description_x000D_ Mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5) is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines. This 471 amino acid protein is widely expressed in tissues. The gene encoding MAPKAPK5 is localized on human chromosome 12q24.12-q24.13._x000D_ Biochem/physiol Actions_x000D_ Mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5) is activated through its phosphorylation by MAP kinases including MAPK1, MAPK14, and MAPK11. It is activated in HeLa cells in response to cellular stress and proinflammatory cytokines. MAPKAPK5 activity is regulated by p38-α and p38-β both in vitro and in vivo, and thr-182 is the regulatory phosphorylation site of MAPKAPK5. In vitro, the protein phosphorylates heat shock protein HSP27 at its physiologically relevant sites. It may have a role in actin polymerization, cell motility and tumor suppression._x000D_ Physical form_x000D_ Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol._x000D_ Preparation Note_x000D_ after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles_x000D_ Legal Information_x000D_ PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC