MRP7 KNOCKOUT CACO-2 CELLS

Description_x000D_ General description_x000D_ The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MRP6 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCC10 (MRP6) efflux transporter._x000D_ Application_x000D_ The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:_x000D_ Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases_x000D_ Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes_x000D_ Caco-2 Transporter Knockout Cell Based Assays_x000D_ Features and Benefits_x000D_ The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:_x000D_ • A functional knockout of the MRP6 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP6 substrate_x000D_ • The vial format enables the MRP6 knockout cells to be included in standard drug transporter protocols_x000D_ • Human assay with no interference from animal inhibitors_x000D_ • Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality_x000D_ Legal Information_x000D_ ADME/Tox Cell Lines License