Description_x000D_ Amino Acid Sequence_x000D_ NFor-Met-Leu-Phe_x000D_ General description_x000D_ N-Formyl-Met-Leu-Phe is a chemotactin peptide, which functions on polymorphonuclear leukocytes (PMN), neutrophils etc._x000D_ Application_x000D_ N-Formyl-Met-Leu-Phe has been used for:_x000D_ • creating gradients in Zigmond chamber chemotaxis assay performed on neutrophils_x000D_ • use in neutrophil chemotaix assay and_x000D_ • determination of the involvement of MAPK-activating protein kinase-2 (MAPKAPK-2) and/or p38, in the signaling pathway of human polymorphonuclear leukocytes (PMNs) stimulated by N-Formyl-Met-Leu-Phe._x000D_ Packaging_x000D_ 5, 10 mg in glass insert_x000D_ 50, 100, 500 mg in glass bottle_x000D_ Biochem/physiol Actions_x000D_ N-Formyl-Met-Leu-Phe induced chemotaxis in phosphoinositide 3-kinase γ (PI3K)γ-/- neutrophils, and promotes adhesion, polymerization of F-actin, Fcγ receptor-mediated phagocytosis and intracellular Ca2+ release. It acts as an inflammatory agent and activates polymorphonuclear leukocytes (PMNs) without the formation of 5-hydroxyicosatetraenoic acid (5-HETE) and leukotriene B4 (LTB4). Its effects on neutrophils and the inhibition of adenylate cyclase are inhibited by pertussis toxin._x000D_ Potent chemotactic peptide. Induces a metabolic burst in macrophages accompanied by an increase in respiratory rate, secretion of lysosomal enzymes, and production of superoxide anion._x000D_ Potent inducer of leucocyte chemotaxis and macrophage activator. Receptors that bind formylpeptides are found on phagocytic neutrophils and have recently been identified on cells of the intestinal mucosa.