Description_x000D_ General description_x000D_ Phospholipids are a class of lipids, which constitute a major component of cell membranes and lipid bilayers. Phospholipids play important roles in signal transduction. The majority of phospholipids contain one diglyceride or sphingosine, a phosphate group, and one choline._x000D_ The Phospholipid quantification assay kit provides a simple and highly sensitive method for measuring choline-containing phospholipids in biological samples. This high-throughput assay can be done within only 30 minutes . In this assay, phospholipids (such as lecithin, lysolecithin, and sphingomyelin) are enzymatically hydrolyzed to release choline, which is determined using choline oxidase and an H2O2 specific dye. The readout is either fluorescence or absorbance, allowing maximal flexibility and a large detection range. The linear range is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance). Phosphatidylcholine-containing phospholipids are present in serum at ~0.2-2.5 mM (~50-200 mg/dL)._x000D_ The detection range of the kit is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance)._x000D_ Application_x000D_ • Measuring choline-containing phospholipids in biological samples (For example: Serum and Plasma)_x000D_ • Enables evaluation of drug effects on phospholipids metabolism_x000D_ Features and Benefits_x000D_ Our economical Phospholipid quantification assay kit also saves your time and efforts:_x000D_ • Highly sensitive, simple and high-throughput assay for measuring choline-containing phospholipids in biological samples_x000D_ • User friendly: No need to weigh or calculate needed amounts of reagents on your own_x000D_ • Supportive calculator (Click here to download a calculator excel file):will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data!_x000D_ • Quick instruction bench card - to assure your experimental success_x000D_ • More experiments in one kit - contains sufficient reagents for 200 tests._x000D_ • Suitable for both colorimetric and fluorometric detection_x000D_ • Detection time: only 30 minutes_x000D_ Suitability_x000D_ Suitable for the detection of phospholipids in biological samples