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Description_x000D_
Application_x000D_
Poly-L-lysine solution has been used for the attachment of endothelial cell cross sections to UV-crosslinked PET membrane frame slides. Poly-L-lysine coated slides were used for the placement of tissue sections for immunohistochemical assay._x000D_
Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule._x000D_
Packaging_x000D_
100, 500 mL in poly bottle_x000D_
Biochem/physiol Actions_x000D_
Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor._x000D_
Components_x000D_
Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties._x000D_
Preparation Note_x000D_
This product is supplied as a 0.10% (w/v) solution in water. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.
- Related Categories Additional Reagents, Adhesives, Amino Acids, Biochemicals and Reagents, Cell Biology, Core Bioreagents, Hematology Stains, Hematology and Histology, Hematology and Histology Lab Accessories, Life Science Reagents for Immunohistochemistry (IHC), Polyamino Acids, Polylysine and Polyornithine Attachment Factors, Research EssentialsMore... Quality Level