PUROMYCIN DIHYDROCHLORIDE

Description_x000D_ General description_x000D_ Chemical structure: peptidyl nucleoside_x000D_ Application_x000D_ Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL._x000D_ Packaging_x000D_ 1 g in glass bottle_x000D_ 25, 100, 250, 500 mg in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3′-terminal end of the aminoacyl-tRNA._x000D_ Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene._x000D_ Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days._x000D_ Features and Benefits_x000D_ This compound is a featured product for Gene Regulation research. Click here to discover more featured Gene Regulation products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm._x000D_ Caution_x000D_ As supplied, this product remains active for four years when stored at -20°C._x000D_ Preparation Note_x000D_ This product is soluble in water at 50 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C. This product is also soluble in methanol at 10 mg/mL._x000D_ Other Notes_x000D_ Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place. Keep in a dry place