Description_x000D_ Application_x000D_ SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining._x000D_ Other Notes_x000D_ Protein (mol. wt.)_x000D_ Aprotinin, bovine lung (6,500)_x000D_ α-Lactalbumin, bovine milk (14,200)_x000D_ Trypsin inhibitor, soybean (20,000)_x000D_ Trypsinogen, bovine pancreas (24,000)_x000D_ Carbonic anhydrase, bovine erythrocytes (29,000)_x000D_ Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)_x000D_ Ovalbumin, chicken egg (45,000)_x000D_ Albumin, bovine serum (66,000)_x000D_ Will yield eight protein bands with Coomassie Blue staining._x000D_ Reconstitution_x000D_ Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 10 mM TCEP, 2% SDS, and 0.01% bromophenol blue._x000D_ Legal Information_x000D_ SigmaMarker is a trademark of Sigma-Aldrich Co. LLC