Description_x000D_ Application_x000D_ Tris-Borate EDTA (TBE) buffer is commonly used in the electrophoresis of nucleic acids in agarose and polyacrylamide gels._x000D_ TBE buffer is recommended for resolution of RNA and DNA fragments smaller than 1500 bp._x000D_ Physical form_x000D_ 10X TBE buffer stock solution contains 0.89 M Trizma(R) with 0.02 M EDTA adjusted to pH 8.3 with boric acid. Dilute with ultrapure water to 1X._x000D_ Notes: If precipitation is present in the 10X concentrated buffer, warm the bottle to 37 °C and mix until completely dissolved prior to dilution._x000D_ It is recommended 1X working solutions be filtered through a 0.2 mm filter before use.