Description General description Triton X-100 has hemolytic property and is used for DNA extraction. Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection. Application Triton™-X has been used: • In the permeabilization of cells for immunofluorescence staining • As a component of lysis buffer in western blot analysis • As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope Packaging 1 L in poly bottle 100, 500 mL in poly bottle 5 gal in poly drum 1 gal in poly bottle 5 mL in glass bottle Biochem/physiol Actions Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions. Legal Information Tri-Chek is a trademark of Micro Essential Laboratory, Inc. Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow