Description_x000D_ General description_x000D_ Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight._x000D_ Application_x000D_ Trizma has been used:_x000D_ • As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells_x000D_ • As a component of pre-hybridization buffer in in situ hybridization_x000D_ • To suspend dried protein before SDS-PAGE protein characterization_x000D_ • As a component of lysis buffer to wash and lyse cells before immunoprecipitation_x000D_ • In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis_x000D_ • In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC_x000D_ • In capillary electrochromatography and UV analysis of tocopherols and tocotrienols_x000D_ Packaging_x000D_ 1 kg in poly bottle_x000D_ 100, 500 g in poly bottle_x000D_ 5 kg in poly drum_x000D_ Other Notes_x000D_ Easily compare specifications for Trizma products with the Trizma specification table._x000D_ The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution._x000D_ For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode._x000D_ Legal Information_x000D_ Trizma is a registered trademark of Sigma-Aldrich Co. LLC